8FZB
Crystal structure of human FAM86A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-10 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 160.701, 160.701, 351.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.820 - 3.350 |
| R-factor | 0.2319 |
| Rwork | 0.231 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.650 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (1.20.1-4487) |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.470 |
| High resolution limit [Å] | 3.350 | 7.210 | 3.350 |
| Rmerge | 0.998 | 0.065 | 1.618 |
| Total number of observations | 1324884 | ||
| Number of reflections | 64154 | 6427 | 6403 |
| <I/σ(I)> | 4.5 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 20.7 | 24.7 | 17.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | 0.1 M Ammonium citrate tribasic (pH 7.0), 10% w/v Polyethylene glycol 3,350 and 5 mM TCEP |
