8FYU
Crystal structure of the human CHIP-TPR domain in complex with a 10mer acetylated tau peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1158 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.340, 46.035, 70.782 |
| Unit cell angles | 90.00, 119.95, 90.00 |
Refinement procedure
| Resolution | 61.330 - 1.848 |
| R-factor | 0.205980463286 |
| Rwork | 0.204 |
| R-free | 0.24364 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.850 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.330 | 1.914 |
| High resolution limit [Å] | 1.848 | 1.848 |
| Rmerge | 1.281 | |
| Rmeas | 1.406 | |
| Number of reflections | 19695 | 1931 |
| <I/σ(I)> | 4.24 | |
| Completeness [%] | 98.5 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.986 | 0.571 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M CaCl2, 0.1 M HEPES (pH 7.4). 28% PEG 4K |
