8FYG

Crystal structure of Hyaluronate lyase A from Cutibacterium acnes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-02-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	51.960, 59.420, 125.990
Unit cell angles	90.61, 95.74, 90.08

Refinement procedure

Resolution	19.800 - 2.050
R-factor	0.2259
Rwork	0.225
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.547
Data reduction software	XDS (VERSION Jun 1, 2017 BUILT=20170923)
Data scaling software	XSCALE (VERSION Jun 1, 2017 BUILT=20170923)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.800	19.800	2.100
High resolution limit [Å]	2.050	9.170	2.050
Rmerge	0.097	0.041	0.497
Rmeas	0.128	0.054	0.662
Number of reflections	88266	895	6191
<I/σ(I)>	7.33
Completeness [%]	93.9
Redundancy	2.2
CC(1/2)	0.991	0.995	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291.15	0.1 M Na dihydrogen phosphate pH 6.5, 9% PEG 8000, 5 mM TCEP