8FXJ
Crystal structure of Fab460
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 129.546, 129.546, 96.808 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.440 - 2.000 |
| R-factor | 0.1678 |
| Rwork | 0.167 |
| R-free | 0.19390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.286 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.102 | 1.193 |
| Rmeas | 0.110 | 1.294 |
| Rpim | 0.041 | 0.489 |
| Number of reflections | 63553 | 3133 |
| <I/σ(I)> | 23.23 | 1.36 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.8 | 6.4 |
| CC(1/2) | 0.992 | 0.571 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 1.5M Lithium sulfate monohydrate, 0.1M Sodium actate, |
