8FWL

Crystal structure of Australian bat lyssavirus nucleoprotein in complex with phosphoprotein chaperone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 2 1
Unit cell lengths	82.373, 35.503, 89.091
Unit cell angles	90.00, 92.63, 90.00

Refinement procedure

Resolution	29.670 - 2.190
R-factor	0.1611
Rwork	0.159
R-free	0.19250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.546
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.670	2.260
High resolution limit [Å]	2.190	2.190
Rmerge	0.076	0.464
Rmeas		0.538
Rpim		0.268
Number of reflections	26863	2185
<I/σ(I)>	10.4	3.3
Completeness [%]	99.5
Redundancy	3.8
CC(1/2)	0.997	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.12M Alcohol mix (0.2M 1,6-Hexanediol; 0.2M 1-Butanol; 0.2M 1,2-Propanediol; 0.2M 2-Propanol; 0.2M 1,4-Butanediol; 0.2M 1,3-Propanediol), 1.0M Tris (base); BICINE,60% Precipitant Mix (40% v/v PEG 500* MME; 20 % w/v PEG 20000)