8FW7

Histone from Bdellovibrio bacteriovorus bound to dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2022-05-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000040
Spacegroup name	I 21 21 21
Unit cell lengths	33.081, 103.143, 111.145
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.790 - 2.000
R-factor	0.2249
Rwork	0.222
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.919
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.790	2.072
High resolution limit [Å]	2.000	2.000
Rmerge	0.060	0.586
Rmeas	0.066	0.630
Rpim	0.025	0.230
Number of reflections	13147	1302
<I/σ(I)>	16.49	3.86
Completeness [%]	98.4	97.89
Redundancy	7.1	7.4
CC(1/2)	0.999	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	15% PEG 550 MME, 50 mM HEPES