8FW1
Gluconobacter Ene-Reductase (GluER) mutant - PagER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE 7B2 |
| Synchrotron site | CHESS |
| Beamline | 7B2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9202 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.165, 51.849, 144.935 |
| Unit cell angles | 90.51, 90.11, 114.86 |
Refinement procedure
| Resolution | 45.510 - 1.500 |
| R-factor | 0.2792 |
| Rwork | 0.268 |
| R-free | 0.27280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6myw |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.760 |
| Data reduction software | DIALS (3.12.1) |
| Phasing software | PHASER (2.0) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.510 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.097 | 0.309 |
| Rpim | 0.077 | 0.239 |
| Number of reflections | 205104 | 10107 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 96.6 | 93.8 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1 M Tris, 0.1 M potassium bromide, 20% w/v PEG4000 |
