8FVX

Histone from Bdellovibrio bacteriovorus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-19
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.541870
Spacegroup name	P 2 21 21
Unit cell lengths	26.772, 34.412, 56.484
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.390 - 1.800
Rwork	0.189
R-free	0.23310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.816
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.390	1.865
High resolution limit [Å]	1.670	1.800
Rmerge	0.052	0.103
Rmeas	0.055	0.114
Rpim	0.019	0.048
Number of reflections	5959	2407
<I/σ(I)>	25.2	10.42
Completeness [%]	86.7	95.25
Redundancy	7.6	5.2
CC(1/2)	0.999	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	3.2 M (NH4)2SO4, 100 mM BICINE