8FVT

Multi-state design of two-state switchable hinge proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99998
Spacegroup name	I 4
Unit cell lengths	67.678, 67.678, 82.374
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.860 - 3.070
R-factor	0.2291
Rwork	0.224
R-free	0.27370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.576
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_4761)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.860	3.280
High resolution limit [Å]	3.070	3.070
Rmerge	0.098	0.415
Rpim	0.145	0.145
Number of reflections	3505	1727
<I/σ(I)>	17.39	5.59
Completeness [%]	99.6	99.48
Redundancy	8.8	9.1
CC(1/2)	1.000	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.8 M Ammonium citrate tribasic pH 7.0