8FUX
KpsC D160C ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.540, 80.300, 65.980 |
| Unit cell angles | 90.00, 103.04, 90.00 |
Refinement procedure
| Resolution | 48.430 - 1.200 |
| R-factor | 0.1254 |
| Rwork | 0.124 |
| R-free | 0.15680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.585 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.430 | 1.250 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmeas | 0.078 | 1.104 |
| Number of reflections | 184570 | 21213 |
| <I/σ(I)> | 9 | 1.39 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 3.3 | 3.2 |
| CC(1/2) | 0.998 | 0.521 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 25 % (v/v) PEG 3350 and 0.1 M Bis-Tris, pH 5.5 |
