8FUU
Crystal structure of Xenopus laevis arrestin 1 - P3221 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.773, 80.773, 120.103 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.560 - 2.890 |
| R-factor | 0.25172 |
| Rwork | 0.249 |
| R-free | 0.29609 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.608 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.070 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.049 | 2.692 |
| Number of reflections | 10542 | 1651 |
| <I/σ(I)> | 21.53 | 0.77 |
| Completeness [%] | 99.8 | |
| Redundancy | 9.9 | |
| CC(1/2) | 1.000 | 0.694 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 25% triethylene glycol 100 mM glycine 100 mM ammonium sulfate |
