8FUT
Crystal structure of Xenopus laevis arrestin 1 - P3121 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 81.830, 81.830, 139.620 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.730 - 2.540 |
| R-factor | 0.19122 |
| Rwork | 0.189 |
| R-free | 0.22912 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.148 | 3.361 |
| Number of reflections | 18443 | 2861 |
| <I/σ(I)> | 13.47 | 0.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 20.1 | |
| CC(1/2) | 0.999 | 0.393 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 140 mM ammonium chloride 70 mM HEPES, pH 7.5 17.5 % glycerol ethoxylate 6 % glycerol |
