8FUQ

Crystal structure of rabies virus (strain CVS-11) P3 dimerization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-15
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9537
Spacegroup name	I 1 2 1
Unit cell lengths	67.050, 44.097, 111.787
Unit cell angles	90.00, 100.44, 90.00

Refinement procedure

Resolution	52.510 - 1.800
R-factor	0.2091
Rwork	0.208
R-free	0.23720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.559
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.530	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.156	1.521
Rpim		2.753
Number of reflections	30167	1797
<I/σ(I)>	2.9	0.4
Completeness [%]	99.9
Redundancy	3.6
CC(1/2)	0.989	0.248

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2 M Sodium nitrate, 0.1 M Bis-Tris propane, 20 % w/v PEG 3350