8FUL
Heterologous AibH1H2 purified from Lysogeny broth
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 82.772, 231.403, 144.997 |
| Unit cell angles | 90.00, 92.17, 90.00 |
Refinement procedure
| Resolution | 47.280 - 2.290 |
| R-factor | 0.1915 |
| Rwork | 0.190 |
| R-free | 0.25790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6m2i |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.056 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.280 | 2.330 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Number of reflections | 119391 | 11704 |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 97.9 | 96.1 |
| Redundancy | 3.1 | 3.1 |
| CC(1/2) | 0.989 | 0.915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.16 M MgCl2, 0.08 M HEPES, 20% PEG4000, 20% glycerol, 10 mM sodium dithionite |
