8FUC

Crystal structure of mouse Importin alpha in complex with Hendra virus matrix protein minor site NLS2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-12
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.550, 90.180, 98.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.620 - 2.100
R-factor	0.177
Rwork	0.175
R-free	0.21690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.618
Data reduction software	MOSFLM (1.19.2_4158)
Data scaling software	Aimless (1.19.2_4158)
Phasing software	PHASER
Refinement software	REFMAC (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.620	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.123
Rpim	0.057	0.302
Number of reflections	41275	3316
<I/σ(I)>	7.2	1.7
Completeness [%]	99.8
Redundancy	5.7
CC(1/2)	0.993	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.72M sodium citrate, 0.1M sodium HEPES, 1% dithiothreitol