8FUA

Crystal structure of mouse Importin alpha in complex with Hendra virus matrix protein NLS1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.488, 89.859, 99.829
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.420 - 1.900
R-factor	0.1737
Rwork	0.173
R-free	0.19000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bw0
RMSD bond length	0.015
RMSD bond angle	1.178
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.420	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.085	1.376
Rpim	0.050	0.820
Number of reflections	56224	26486
<I/σ(I)>	11.9
Completeness [%]	99.9	100
Redundancy	7.2	7.1
CC(1/2)	0.998	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M sodium HEPES, 0.72M sodium citrate, 1% dithiothreitol