8FTT

Crystal structure of a glycosylase specific nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 2
Unit cell lengths	121.806, 76.022, 75.767
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.260 - 2.080
R-factor	0.2523
Rwork	0.199
R-free	0.22890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.484
Data reduction software	SAINT (V8.40B)
Data scaling software	Aimless (0.7.8)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.270	2.140
High resolution limit [Å]	2.080	2.080
Rmerge	0.120	1.825
Rmeas	0.130	1.968
Rpim	0.049	0.724
Number of reflections	42462	3094
<I/σ(I)>	10.6	1.3
Completeness [%]	99.3	93.9
Redundancy	6.9	7
CC(1/2)	0.998	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	287	0.1 M sodium citrate pH 5.6, 1.2 M lithium sulfate, and 0.5 M ammonium sulfate