8FTR
SgvM methyltransferase with MTA and alpha-ketoleucine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-08-02 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.134, 67.134, 184.338 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.270 - 2.130 |
| R-factor | 0.2124 |
| Rwork | 0.211 |
| R-free | 0.24560 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.003 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.300 | 2.136 |
| High resolution limit [Å] | 2.129 | 2.129 |
| Rmerge | 0.098 | |
| Number of reflections | 23766 | 4532 |
| <I/σ(I)> | 23.3 | |
| Completeness [%] | 96.8 | |
| Redundancy | 19.5 | |
| CC(1/2) | 1.000 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 283 | PEG 3350 pH 7.5-8.5 |
