8FTR

SgvM methyltransferase with MTA and alpha-ketoleucine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-08-02
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97856
Spacegroup name	P 43 21 2
Unit cell lengths	67.134, 67.134, 184.338
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.270 - 2.130
R-factor	0.2124
Rwork	0.211
R-free	0.24560
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.003
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.300	2.136
High resolution limit [Å]	2.129	2.129
Rmerge	0.098
Number of reflections	23766	4532
<I/σ(I)>	23.3
Completeness [%]	96.8
Redundancy	19.5
CC(1/2)	1.000	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		283	PEG 3350 pH 7.5-8.5