8FTM
Setx-ssRNA-ADP-SO4 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-16 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.705, 107.323, 162.029 |
| Unit cell angles | 90.00, 99.64, 90.00 |
Refinement procedure
| Resolution | 44.540 - 3.010 |
| R-factor | 0.2139 |
| Rwork | 0.212 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mzn |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.524 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.121 | 0.725 |
| Number of reflections | 37676 | 1866 |
| <I/σ(I)> | 11.42 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.999 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 200 mM sodium sulfate, 20% w/v PEG3350 |
