8FTB

Crystal structure of integrin beta-2 tail bound to the FERM-folded talin head domain with a K306Q mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92011
Spacegroup name	P 21 2 21
Unit cell lengths	67.006, 68.394, 110.617
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.090 - 1.970
R-factor	0.1846
Rwork	0.183
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.978
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.370	2.020
High resolution limit [Å]	1.970	1.970
Rmerge	0.150	0.819
Number of reflections	36669	2559
<I/σ(I)>	8.7
Completeness [%]	99.6
Redundancy	6.9
CC(1/2)	0.994	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	PEG4000 15%, NaCl 0.1M, DTT 2mM