8FT9
Crystal structure of a new antigen receptor variable domain from nurse sharks
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.824, 89.891, 186.849 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.700 - 2.740 |
| R-factor | 0.2414 |
| Rwork | 0.240 |
| R-free | 0.27750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.464 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.700 | 2.838 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.365 | 1.022 |
| Rmeas | 0.402 | |
| Number of reflections | 26818 | 2472 |
| <I/σ(I)> | 4.23 | |
| Completeness [%] | 96.7 | 96.1 |
| Redundancy | 5.98 | 6.5 |
| CC(1/2) | 0.965 | 0.792 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 20 percent PEG 8000 and 0.2 M potassium nitrate. HT crystallization screen Wizard PEG-Ion 48 Salt with PEG 8K and 10K from Molecular Dimensions |
