8FT1

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2021-02-26
Detector	OXFORD TITAN CCD
Wavelength(s)	1.54056
Spacegroup name	P 43 21 2
Unit cell lengths	78.433, 78.433, 36.955
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.550 - 1.600
R-factor	0.22927
Rwork	0.227
R-free	0.27121
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.497
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.550	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.077	0.507
Rmeas	0.086	0.618
Rpim	0.036	0.346
Total number of observations	75409	2302
Number of reflections	15714	760
<I/σ(I)>	10.3	1.2
Completeness [%]	99.7
Redundancy	4.8	3
CC(1/2)	0.992	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection