8FSU

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2020-12-14
Detector	OXFORD TITAN CCD
Wavelength(s)	1.5406
Spacegroup name	P 43 21 2
Unit cell lengths	77.235, 77.235, 37.159
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.380 - 1.600
R-factor	0.21363
Rwork	0.212
R-free	0.25552
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qwy
RMSD bond length	0.008
RMSD bond angle	1.454
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.380	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.154	2.147
Rmeas	0.172	2.659
Rpim	0.074	1.530
Total number of observations	69307	2156
Number of reflections	15190	739
<I/σ(I)>	5.6	0.8
Completeness [%]	98.8
Redundancy	4.6	2.9
CC(1/2)	0.990	0.288

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection