8FSN

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2020-12-09
Detector	OXFORD TITAN CCD
Wavelength(s)	1.5406
Spacegroup name	P 43 21 2
Unit cell lengths	78.104, 78.104, 37.135
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.510 - 1.600
R-factor	0.22408
Rwork	0.222
R-free	0.26296
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qwy
RMSD bond length	0.008
RMSD bond angle	1.475
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.510	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.108	0.808
Rmeas	0.120	0.991
Rpim	0.051	0.565
Total number of observations	70764	2149
Number of reflections	15673	752
<I/σ(I)>	7.3	1
Completeness [%]	99.8
Redundancy	4.5	2.9
CC(1/2)	0.994	0.485

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection