8FSH

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2020-11-09
Detector	OXFORD TITAN CCD
Wavelength(s)	1.54056
Spacegroup name	P 43 21 2
Unit cell lengths	77.315, 77.315, 37.085
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.390 - 1.600
R-factor	0.19533
Rwork	0.193
R-free	0.23754
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.605
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.390	1.500
High resolution limit [Å]	1.480	1.480
Rmerge	0.086	2.426
Rmeas	0.097	3.367
Rpim	0.041	2.326
Total number of observations	80283	321
Number of reflections	17446	222
<I/σ(I)>	9.8	0.8
Completeness [%]	89.8
Redundancy	4.6	1.4
CC(1/2)	0.996	0.030

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection