8FSG

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2020-11-06
Detector	OXFORD TITAN CCD
Wavelength(s)	1.54056
Spacegroup name	P 43 21 2
Unit cell lengths	77.176, 77.176, 37.241
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.380 - 1.600
R-factor	0.19965
Rwork	0.197
R-free	0.24580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.575
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.380	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.067	0.550
Rmeas	0.076	0.675
Rpim	0.033	0.382
Total number of observations	67239	1949
Number of reflections	15059	688
<I/σ(I)>	11.3	1.6
Completeness [%]	98.0
Redundancy	4.5	2.8
CC(1/2)	0.998	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection