8FS0

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2020-11-02
Detector	OXFORD TITAN CCD
Wavelength(s)	1.5406
Spacegroup name	P 43 21 2
Unit cell lengths	78.264, 78.264, 37.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.530 - 1.600
R-factor	0.24409
Rwork	0.242
R-free	0.28667
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qwy
RMSD bond length	0.010
RMSD bond angle	1.627
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.530	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.093	3.165
Rmeas	0.105	3.921
Rpim	0.047	2.262
Total number of observations	69666	2084
Number of reflections	15539	728
<I/σ(I)>	8	0.3
Completeness [%]	99.1
Redundancy	4.5	2.9
CC(1/2)	0.996	0.207

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection