8FRQ

LSD1-CoREST in complex with T14, long soaking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.965459
Spacegroup name	I 2 2 2
Unit cell lengths	119.331, 179.769, 234.464
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.710 - 2.890
R-factor	0.2183
Rwork	0.217
R-free	0.24090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.478
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.710	2.970
High resolution limit [Å]	2.890	2.890
Rmerge	0.152	1.172
Rmeas	0.182	1.349
Rpim	0.096	0.648
Total number of observations	221726	19375
Number of reflections	54900	4539
<I/σ(I)>	8.5	1.5
Completeness [%]	97.1
Redundancy	4	4.3
CC(1/2)	0.809	0.374

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1.2 Na/K Tartrate, 100 mM ADA pH 6.5