8FRF

Homodimeric designed loop protein RBL7_C2_3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	.9795
Spacegroup name	P 21 21 21
Unit cell lengths	111.961, 117.644, 142.056
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.970 - 2.990
R-factor	0.2122
Rwork	0.210
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.692
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.970	3.097
High resolution limit [Å]	2.990	2.990
Rmeas	0.360	3.294
Rpim	0.138	1.250
Number of reflections	38604	3796
<I/σ(I)>	5.35	0.66
Completeness [%]	99.4	96.63
Redundancy	6.7	6.8
CC(1/2)	0.996	0.407

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	2.4 M sodium malonate, pH 7.0