8FQJ
LSD1-CoREST in complex with T14, short soaking
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-22 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.965459 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 118.883, 179.340, 233.389 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.400 - 2.900 |
| R-factor | 0.2188 |
| Rwork | 0.218 |
| R-free | 0.24560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.534 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.560 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.143 | 1.625 |
| Rmeas | 0.163 | 1.872 |
| Rpim | 0.076 | 0.902 |
| Total number of observations | 224031 | 17067 |
| Number of reflections | 54215 | 4399 |
| <I/σ(I)> | 7.8 | 0.9 |
| Completeness [%] | 97.9 | |
| Redundancy | 4.1 | 3.9 |
| CC(1/2) | 0.993 | 0.280 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.2 Na/K Tartrate, 100 mM ADA pH 6.5 |
