8FPO
PCSK9 in complex with an inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-17 |
| Detector | DECTRIS EIGER R 1M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.952, 70.751, 150.093 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.053 - 2.997 |
| R-factor | 0.185 |
| Rwork | 0.182 |
| R-free | 0.24990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.665 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | FFT |
| Refinement software | PHENIX (1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.093 | 3.007 |
| High resolution limit [Å] | 2.997 | 2.997 |
| Rmerge | 0.165 | 0.543 |
| Number of reflections | 14084 | 147 |
| <I/σ(I)> | 9.9 | 3.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris-HCl (pH 8.0), 14-19% PEG 6000 |
