8FPN
Crystal structure of hen egg white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 125 |
| Detector technology | CCD |
| Collection date | 2020-09-01 |
| Detector | OXFORD TITAN CCD |
| Wavelength(s) | 1.54056 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.462, 77.462, 37.136 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.000 - 1.600 |
| R-factor | 0.23943 |
| Rwork | 0.237 |
| R-free | 0.29188 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.537 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.400 | 1.500 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.072 | 7.889 |
| Rmeas | 0.081 | 9.993 |
| Rpim | 0.034 | 6.016 |
| Total number of observations | 258 | |
| Number of reflections | 16740 | 111 |
| <I/σ(I)> | 9.3 | 0.1 |
| Completeness [%] | 85.6 | |
| Redundancy | 4.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.6 | 295 | 1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection |
