8FPD

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2020-08-27
Detector	OXFORD TITAN CCD
Wavelength(s)	1.54056
Spacegroup name	P 43 21 2
Unit cell lengths	78.130, 78.130, 36.962
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.510 - 1.600
R-factor	0.24904
Rwork	0.247
R-free	0.29068
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.676
Data reduction software	HKL-2000
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.510	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.113	0.745
Rmeas	0.126	0.916
Rpim	0.053	0.521
Total number of observations	71424	2112
Number of reflections	15438	740
<I/σ(I)>	7.1	0.6
Completeness [%]	99.0
Redundancy	4.6	2.9
CC(1/2)	0.975	0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection