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8FP7

Crystal structure of hen egg white lysozyme

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSEALED TUBE
Source detailsOXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]125
Detector technologyCCD
Collection date2020-08-13
DetectorOXFORD TITAN CCD
Wavelength(s)1.54056
Spacegroup nameP 43 21 2
Unit cell lengths78.362, 78.362, 37.068
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution13.550 - 1.600
R-factor0.20911
Rwork0.207
R-free0.24487
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.009
RMSD bond angle1.614
Data reduction softwareHKL-2000
Data scaling softwareAimless
Refinement softwareREFMAC (5.8.0238)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]13.55013.550
High resolution limit [Å]1.6001.600
Rmerge0.048
Number of reflections1544315439
<I/σ(I)>17.5
Completeness [%]98.2
Redundancy4.5
CC(1/2)0.999
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1EVAPORATION4.62951.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection

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