8FP6
Crystal structure of hen egg white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 125 |
| Detector technology | CCD |
| Collection date | 2020-08-12 |
| Detector | OXFORD TITAN CCD |
| Wavelength(s) | 1.54056 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.511, 78.511, 37.037 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.570 - 1.600 |
| R-factor | 0.20165 |
| Rwork | 0.200 |
| R-free | 0.22813 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.650 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.570 | 13.570 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.069 | |
| Number of reflections | 15619 | 15619 |
| <I/σ(I)> | 13 | |
| Completeness [%] | 99.1 | |
| Redundancy | 4.9 | |
| CC(1/2) | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.6 | 295 | 1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection |
