8FON

Crystal structure of tRNA^Lys(SUU) bound to AUA codon in the ribosomal P site

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978900
Spacegroup name	P 21 21 21
Unit cell lengths	210.560, 447.230, 618.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	174.010 - 3.640
R-factor	0.2336
Rwork	0.232
R-free	0.27820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	174.010	174.010	3.590
High resolution limit [Å]	3.500	15.650	3.500
Rmerge	0.214	0.029	1.471
Rmeas	0.242	0.033	1.660
Total number of observations	3043425
Number of reflections	696830	7871	52193
<I/σ(I)>	6.51	32.01	1.04
Completeness [%]	95.9	90	97.8
Redundancy	4.368	4.394	4.443
CC(1/2)	0.997	0.999	0.276

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP