8FNR

X-ray crystal structure of Hansschlegelia quercus lanmodulin (LanM) with dysprosium (III) bound at pH 7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2022-07-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	56.000, 54.234, 65.275
Unit cell angles	90.00, 93.57, 90.00

Refinement procedure

Resolution	27.410 - 1.400
R-factor	0.1619
Rwork	0.161
R-free	0.18080
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	138347	8026
<I/σ(I)>	27.972
Completeness [%]	91.6
Redundancy	7.5
CC(1/2)	0.997	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294	250 uM tri-sodium citrate, pH 7, and 27 % (w/v) PEG 6000