8FMR
Complex structure of K210 deletion Troponin complex with ibandronate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.531, 169.315, 69.413 |
| Unit cell angles | 90.00, 102.12, 90.00 |
Refinement procedure
| Resolution | 43.393 - 3.238 |
| R-factor | 0.2647 |
| Rwork | 0.261 |
| R-free | 0.32950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.532 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.173 | 0.801 |
| Number of reflections | 13370 | 1171 |
| <I/σ(I)> | 9.1 | 1.4 |
| Completeness [%] | 95.9 | |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M Sodium Acetate, 20% PEG3350 |
