8FMN
Complex structure of K210 deletion Troponin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97973 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.272, 170.503, 69.462 |
| Unit cell angles | 90.00, 101.72, 90.00 |
Refinement procedure
| Resolution | 43.612 - 3.101 |
| R-factor | 0.2401 |
| Rwork | 0.239 |
| R-free | 0.26590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.486 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.076 | 0.458 |
| Number of reflections | 15584 | 1342 |
| <I/σ(I)> | 17.7 | 2.7 |
| Completeness [%] | 94.2 | |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M sodium acetate, 20% PEG3350 |
