8FM8

HIV-1 gp120 complex with CJF-IV-046

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-17
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	71.534, 121.790, 194.603
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.650 - 2.470
R-factor	0.2193
Rwork	0.217
R-free	0.26250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.669
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.650	2.740
High resolution limit [Å]	2.470	2.470
Rmerge	0.250	1.877
Rpim	0.070	0.523
Number of reflections	42968	2148
<I/σ(I)>	10.3
Completeness [%]	94.3
Redundancy	13.6
CC(1/2)	0.996	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	14% to 16% (w/v) PEG 1500, 0.1 M calcium chloride, 0.1 M imidazole pH 6.5