8FM7

HIV-1 gp120 complex with CJF-III-192

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-17
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	72.360, 121.100, 195.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.780 - 2.340
R-factor	0.2383
Rwork	0.237
R-free	0.27130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.700
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.780	2.630
High resolution limit [Å]	2.340	2.340
Rmerge	0.256	1.509
Rpim	0.077	0.475
Number of reflections	49263	2738
<I/σ(I)>	8.5
Completeness [%]	94.5
Redundancy	12.1
CC(1/2)	0.994	0.657

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	14% to 16% (w/v) PEG 1500, 0.1 M calcium chloride, 0.1 M imidazole pH 6.5