8FM3

HIV-1 gp120 complex with CJF-III-288

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-12
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	71.464, 121.718, 195.183
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.800 - 2.110
R-factor	0.2479
Rwork	0.247
R-free	0.27290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.403
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.800	2.410
High resolution limit [Å]	2.110	2.110
Rmerge	0.168	1.298
Rpim	0.070	0.544
Number of reflections	55435	2771
<I/σ(I)>	8.6
Completeness [%]	99.6
Redundancy	6.7
CC(1/2)	0.996	0.572

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	14% to 16% (w/v) PEG 1500, 0.1 M calcium chloride, 0.1 M imidazole pH 6.5