8FM0

HIV-1 gp120 complex with CJF-III-214

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-17
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	71.796, 120.888, 195.163
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.790 - 2.080
R-factor	0.2536
Rwork	0.253
R-free	0.27330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.510
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.790	2.300
High resolution limit [Å]	2.080	2.080
Rmerge	0.135	1.176
Rpim		0.469
Number of reflections	501236	3829
<I/σ(I)>	9.9
Completeness [%]	93.5
Redundancy	6.9
CC(1/2)	0.998	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	14% to 16% (w/v) PEG 1500, 0.1 M calcium chloride, 0.1 M imidazole pH 6.5