8FK3

Adeno-Associated Virus Porcine Origin capsid protein basic regions in complex with Importin-alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.149, 89.457, 99.394
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.600
R-factor	0.2139
Rwork	0.212
R-free	0.24950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oih
RMSD bond length	0.003
RMSD bond angle	0.572
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.960	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.081	0.643
Number of reflections	21468	2616
<I/σ(I)>	9.6
Completeness [%]	97.9
Redundancy	3.9
CC(1/2)	0.997	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.75M Na Citrate, 0.01M DTT, 0.1M HEPES pH 6.5