8FJG

The two-repeat design H12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-15
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.98197
Spacegroup name	P 21 21 21
Unit cell lengths	30.456, 46.595, 73.458
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.490 - 2.130
R-factor	0.2589
Rwork	0.258
R-free	0.27990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.904
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.490	2.210
High resolution limit [Å]	2.130	2.130
Rmerge	0.167	0.167
Rpim	0.051	0.051
Number of reflections	11009	546
<I/σ(I)>	11.03	0.92
Completeness [%]	98.3
Redundancy	12.2
CC(1/2)	0.992	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.09 M sodium fluoride, 0.09 M sodium bromide, 0.09 sodium iodide, 0.0499 M HEPES, 0.0501 M MOPS (acid), 12.5% v/v MPD, 12.5% PEG 1000, and 12.5% w/v PEG 3350