8FJD

Structure of chlorophyllase from Triticum aestivum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2022-04-07
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	P 65 2 2
Unit cell lengths	197.520, 197.520, 96.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.130 - 4.460
R-factor	0.2662
Rwork	0.266
R-free	0.27810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.591
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.620
High resolution limit [Å]	4.460	4.460
Number of reflections	6846	684
<I/σ(I)>	7.44
Completeness [%]	96.8
Redundancy	12.57
CC(1/2)	0.995	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M calcium acetate, 0.1 M sodium cacodylate (pH 6.5), 40% PEG300