8FJ7
LSD1-CoREST in complex with T108 and SNAG peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-02 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.999998 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 119.536, 179.040, 234.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.580 - 2.800 |
| R-factor | 0.2224 |
| Rwork | 0.222 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.547 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.010 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.123 | 4.572 |
| Rmeas | 0.133 | 4.933 |
| Rpim | 0.051 | 1.843 |
| Total number of observations | 420561 | 31856 |
| Number of reflections | 61982 | 4518 |
| <I/σ(I)> | 10.9 | 0.5 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.8 | 7.1 |
| CC(1/2) | 0.999 | 0.237 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.2 Na/K Tartrate, 100 mM ADA pH 6.5 |
