8FJ4

LSD1-CoREST in complex with T108, short soaking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.96546
Spacegroup name	I 2 2 2
Unit cell lengths	118.346, 179.743, 232.329
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.780 - 2.760
R-factor	0.2247
Rwork	0.224
R-free	0.24770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.444
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.780	2.830
High resolution limit [Å]	2.760	2.760
Rmerge	0.116	2.626
Rmeas	0.134	3.019
Rpim	0.065	1.453
Total number of observations	251831	16362
Number of reflections	63077	4079
<I/σ(I)>	8.3	0.5
Completeness [%]	99.0
Redundancy	4	4
CC(1/2)	0.996	0.202

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1.2 Na/K Tartrate, 100 mM ADA pH 6.5