8FJ2

Crystal Structure of the Tick Evasin EVA-AAM1001(C8) Complexed to Human Chemokine CCL17

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953
Spacegroup name	I 2 2 2
Unit cell lengths	60.641, 62.536, 92.684
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.530 - 2.070
R-factor	0.214
Rwork	0.213
R-free	0.23070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.211
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.340	2.130
High resolution limit [Å]	2.070	2.070
Number of reflections	10995	785
<I/σ(I)>	8.7
Completeness [%]	99.3
Redundancy	12.7
CC(1/2)	0.992	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	4.3 M NaCl, 0.1 M HEPES pH 7.4