8FIQ

Multi-state design of two-state switchable hinge proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	71.882, 71.882, 127.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.720 - 2.660
R-factor	0.272
Rwork	0.268
R-free	0.30610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.361
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.720	2.800
High resolution limit [Å]	2.660	2.660
Rmerge	0.136	4.340
Rpim	0.038	1.220
Number of reflections	9942	1246
<I/σ(I)>	11.84
Completeness [%]	97.9	89.38
Redundancy	13.9
CC(1/2)	1.000	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Magnesium sulfate, 20% (w/v) PEG 3350